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Literature summary extracted from

  • Sobrado, P.; Fitzpatrick, P.F.
    Analysis of the role of the active site residue Arg98 in the flavoprotein tryptophan 2-monooxygenase, a member of the L-amino oxidase family (2003), Biochemistry, 42, 13826-13832.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.3 R98A decrease in Vmax, increase in Km-value of tryptophan Pseudomonas savastanoi
1.13.12.3 R98K decrease in Vmax, increase in Km-value of tryptophan Pseudomonas savastanoi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.3 3-Indoleacetamide
-
 Pseudomonas savastanoi
1.13.12.3 3-indolepyruvate
-
 Pseudomonas savastanoi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.3 0.04
-
 tryptophan pH 8.3, 25°C, wild-type Pseudomonas savastanoi
1.13.12.3 0.067
-
 O2 pH 8.3, 25°C, mutant R98A Pseudomonas savastanoi
1.13.12.3 0.09
-
 O2 pH 8.3, 25°C, wild-type Pseudomonas savastanoi
1.13.12.3 0.14
-
 O2 pH 8.3, 25°C, mutant R98K Pseudomonas savastanoi
1.13.12.3 3.3
-
 tryptophan pH 8.3, 25°C, mutant R98A Pseudomonas savastanoi
1.13.12.3 30
-
 tryptophan pH 8.3, 25°C, mutant R98K Pseudomonas savastanoi

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.3 Pseudomonas savastanoi
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.3 L-tryptophan + O2
-
 Pseudomonas savastanoi indole-3-acetamide + CO2 + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.3 0.21
-
 tryptophan pH 8.3, 25°C, mutant R98A Pseudomonas savastanoi
1.13.12.3 1.05
-
 tryptophan pH 8.3, 25°C, mutant R98K Pseudomonas savastanoi
1.13.12.3 13.2
-
 tryptophan pH 8.3, 25°C, wild-type Pseudomonas savastanoi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.12.3 16
-
 3-Indoleacetamide pH 8.3, 25°C, wild-type Pseudomonas savastanoi
1.13.12.3 20
-
 3-Indoleacetamide pH 8.3, 25°C, mutant R98K Pseudomonas savastanoi
1.13.12.3 40
-
 3-indolepyruvate pH 8.3, 25°C, wild-type Pseudomonas savastanoi
1.13.12.3 45
-
 3-Indoleacetamide pH 8.3, 25°C, mutant R98A Pseudomonas savastanoi
1.13.12.3 471
-
 3-indolepyruvate pH 8.3, 25°C, mutant R98K Pseudomonas savastanoi
1.13.12.3 670
-
 3-indolepyruvate pH 8.3, 25°C, mutant R98A Pseudomonas savastanoi